ZNF703 Antibody (CL0654) - Azide and BSA Free

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: ZNF703 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Analysis in human fallopian tube and liver tissues using NBP3-44019 antibody. Corresponding ZNF703 RNA-seq data are presented for the same tissues.

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ZNF703 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0654
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ZNF703 Antibody (CL0654) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZNF703 Antibody (CL0654) - Azide and BSA Free (NBP2-52940) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H7S9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF703
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ZNF703 Antibody (CL0654) - Azide and BSA Free

• FLJ14299
• ZEPPO1
• Zinc finger elbow-related proline domain protein 1
• zinc finger protein 703
• ZNF503L
• ZPO1

Background

ZNF703 interacts selectively with zinc (Zn) ions. May function as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF703