ZNF313 Antibody - BSA Free

Western Blot: ZNF313 Antibody [NBP1-87204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunocytochemistry/Immunofluorescence: ZNF313 Antibody [NBP1-87204] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Western Blot: ZNF313 Antibody [NBP1-87204] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF313 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF313 Antibody - BSA Free (NBP1-87204) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ZNF313 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF114
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ZNF313 Protein (NBP1-87204PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ZNF313 Antibody - BSA Free

• ring finger protein 114
• Zinc finger protein 228
• Zinc finger protein 313ZNF313PSORS12
• ZNF228

Background

ZNF313 is a novel human zinc finger protein gene that contains both ring finger and C(2)H(2) domain. It may play a role in spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF313 Antibody (NBP1-87204)(1)

Publication using NBP1-87204

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: RNF114