N4BP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: N4BP1 Antibody [NBP1-76531] - Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 ug/mL.

Western Blot: N4BP1 Antibody - BSA Free [NBP1-76531] - WB Validation in Human Cell LinesLoading: 15 ug of lysates Antibodies: N4BP1, 1 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 sec

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531] - Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

N4BP1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1. Amino Acid Squence: TAPAEKAELLEQSRGRIE
• Specificity: At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: N4BP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 0.5 ug/ml
• Control Peptide:

  N4BP1 Peptide (NBP1-76531PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for N4BP1 Antibody - BSA Free

• FLJ31821
• KIAA0615
• MGC176730
• NEDD4 binding protein 1
• NEDD4-binding protein 1

Background

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: N4BP1
• Uniprot:
  • NP_694574(Human)