N4BP1 Antibody - BSA Free

Images

 
Western Blot: N4BP1 Antibody [NBP1-76531] - HeLa cell lysate with N4BP1 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody [NBP1-76531] - Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody [NBP1-76531] - Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

N4BP1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1. Amino Acid Squence: TAPAEKAELLEQSRGRIE
Specificity
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N4BP1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Western Blot 0.5 ug/ml
Control Peptide
N4BP1 Peptide (NBP1-76531PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for N4BP1 Antibody - BSA Free

  • FLJ31821
  • KIAA0615
  • MGC176730
  • NEDD4 binding protein 1
  • NEDD4-binding protein 1

Background

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for N4BP1 Antibody (NBP1-76531)

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Bioinformatics

Gene Symbol N4BP1
Uniprot