ZNF281 Antibody - BSA Free

Western Blot: ZNF281 Antibody [NBP1-76551] - Analysis in A-20 cell lysate with antibody at 0.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ZNF281 Antibody - BSA Free [NBP1-76551] - Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZNF281 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF281 Antibody - BSA Free (NBP1-76551) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281. The immunogen is located within amino acids 600 - 650 of ZFP281. Amino Acid Squence: YSNKSESQKEDPFNIAEPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF281
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 0.5 ug/ml
• Control Peptide:

  ZNF281 Peptide (NBP1-76551PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZNF281 Antibody - BSA Free

• FLJ20557
• GZP1
• ZBP-99
• zinc finger protein 416
• ZNF281
• ZNP-99

Background

ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF281
• Uniprot:
  • AAH51905(Human)