ZNF185 Antibody - BSA Free

Immunohistochemistry-Paraffin: ZNF185 Antibody [NBP1-86452] - Analysis in human epididymis and pancreas tissues using NBP1-86452 antibody. Corresponding ZNF185 RNA-seq data are presented for the same tissues.

Staining of human epididymis, liver, pancreas and skeletal muscle using Anti-ZNF185 antibody NBP1-86452 (A) shows similar protein distribution across tissues to independent antibody NBP1-86192 (B).

Immunohistochemistry-Paraffin: ZNF185 Antibody [NBP1-86452] - Staining of human panceas shows no positivity in exocrine glandular cells as expected.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human epididymis shows strong membranous positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Liver tissue

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF185 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF185 Antibody - BSA Free (NBP1-86452) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: HSYVLSAAKKSTGSPTQETQAPFIAKRVEVVEEDGPSEKSQDPPALARSTPGSNSSRGEEIVRLQILTPRAGLRLVAPDVEGMRSSPGNKDKEAPCSRELQRDLAGEEAFRAPNTDAARSSAQLSDGNVGSGATGSRPEGLAAVDIGSERGSSSATSVSAAPADRKSNSTAAQEDAKAD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF185
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ZNF185 Protein (NBP1-86452PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ZNF185 Antibody - BSA Free

• zinc finger protein 185 (LIM domain)

Background

ZNF185 encodes a LIM-domain zinc finger protein. These proteins are thought to be involved in protein-protein interactions.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF185 Antibody (NBP1-86452)(1)

Publication using NBP1-86452

• Huang Z, You Y, Qiu Q et al. Iron-responsive ZNF185 overexpression drives mitochondrial fission and endoplasmic reticulum stress via cytoskeletal remodeling in granulosa cells. Cell Death Discovery 2025-09-15 [PMID: 40877235]

Bioinformatics

• Gene Symbol: ZNF185