ZNF179 Antibody - BSA Free

Western Blot: ZNF179 Antibody [NBP2-13236] - Analysis in control (vector only transfected HEK293T lysate) and RNF112 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: ZNF179 Antibody [NBP2-13236] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear speckles.

Immunohistochemistry-Paraffin: ZNF179 Antibody [NBP2-13236] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Analysis in control (vector only transfected HEK293T lysate) and RNF112 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF179 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: LAQEIKNLSGWMGRTGPGFTSPDEMAAQLHDLRKVEAAKREFEEYVRQQDVATKRIFSALRVLPDTMRNLLSTQKDAILARHGVALLCKGRDQTLEALEAELQATAKAFMDSYT
• Predicted Species: Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF112
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ZNF179 Protein (NBP2-13236PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ZNF179 Antibody - BSA Free

• BFPZinc finger protein 179ZNF179Brain finger protein
• FLJ99413
• ring finger protein 112

Background

ZNF179 encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF179 Antibody (NBP2-13236)(1)

Publication using NBP2-13236

• Lee KH, Chen CL, Lee YC et al. Znf179 induces differentiation and growth arrest of human primary glioblastoma multiforme in a p53-dependent cell cycle pathway Sci Rep 2017-07-06 [PMID: 28684796] (IHC-P, Human)

Bioinformatics

• Gene Symbol: RNF112