ZNF148 Antibody

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Western Blot: ZNF148 Antibody [NBP1-71872] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at for WB at 0.1 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

ZNF148 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 744 and 794 of human Zinc Finger DNA-Binding Protein 89 using the numbering given in entry NP_068799.2 (GeneID 7707).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF148
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZNF148 Antibody

  • BERF-1
  • BFCOL1
  • CACCC box-binding protein
  • CLL-associated antigen KW-10
  • HT-BETA
  • pHZ-52
  • Transcription factor ZBP-89
  • ZBP89
  • ZBP-89
  • ZFP148zinc finger protein 148 (pHZ-52)
  • Zinc finger DNA-binding protein 89
  • zinc finger protein 148

Background

ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZNF148
Entrez
Uniprot