Zfp219 Antibody - BSA Free

Western Blot: Zfp219 Antibody [NBP1-76550] - Analysis in EL4 cell lysate with antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: Zfp219 Antibody - BSA Free [NBP1-76550] - Immunofluorescence of Zfp219 in mouse brain tissue with Zfp219 antibody at 20 ug/mL.

Immunohistochemistry: Zfp219 Antibody - BSA Free [NBP1-76550] - Immunohistochemistry of Zfp219 in mouse brain tissue with Zfp219 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Zfp219 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219. Amino Acid Squence: SHLRTHQPERPRSPAARL
• Specificity: At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF219
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Zfp219 Peptide (NBP1-76550PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Zfp219 Antibody - BSA Free

• FLJ14457
• MGC126229
• MGC126230
• zinc finger protein 514

Background

ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Zfp219 Antibody (NBP1-76550)(1)

Publication using NBP1-76550

• Yuan P, Wang S, Du T et al. ZNF219, a novel transcriptional repressor, inhibits transcription of the prototype foamy virus by interacting with the viral LTR promoter Virus research 2023-06-29 [PMID: 37356580] (WB, Human)

Bioinformatics

• Gene Symbol: ZNF219
• Uniprot:
  • NP_001095142(Human)