ZCCHC11/TUT4 Antibody

Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)

Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZCCHC11/TUT4 Antibody Summary

• Description: Novus Biologicals Goat ZCCHC11/TUT4 Antibody (NB100-783) is a polyclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-ZCCHC11/TUT4 Antibody: Cited in 17 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RERCPHPPRGNVSE corresponding to C-Terminus according to NP_001009881.1, NP_056084.1, NP_001009882.1.
• Specificity: This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TUT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 - 6 ug/mL
  • Immunohistochemistry-Paraffin 2 - 6 ug/mL
  • Peptide ELISA Detection limit 1:16000
• Application Notes: IHC-P: Human Lymph Node shows nuclear staining of select lymphocytes and staining of macrophages.
• Control:

  Raji Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZCCHC11/TUT4 Antibody

• DKFZp779C1943
• EC 2.7.7.52
• KIAA0191FLJ42878
• PAP associated domain containing 3
• PAPD3
• terminal uridylyltransferase 4
• TUT4
• TUTase 4
• Zinc finger CCHC domain-containing protein 11
• zinc finger, CCHC domain containing 11

Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZCCHC11/TUT4 Antibody (NB100-783)(17)

Publications using NB100-783

• E Goy, M Tomezak, C Facchin, N Martin, E Bouchaert, J Benoit, C de Schutte, J Nassour, L Saas, C Drullion, PM Brodin, A Vandeputte, O Molendi-Co, L Pineau, G Goormachti, O Pluquet, A Pourtier, F Cleri, E Lartigau, N Penel, C Abbadie The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion Elife, 2022-03-18;11(0):. 2022-03-18 [PMID: 35302491]
• Nicole Berndt, Christine Wolf, Kristina Fischer, Emanuel Cura Costa, Peter Knuschke, Nick Zimmermann, Franziska Schmidt, Martin Merkel, Osvaldo Chara, Min Ae Lee-Kirsch, Claudia Günther Photosensitivity and cGAS-Dependent IFN-1 Activation in Patients with Lupus and TREX1 Deficiency. The Journal of investigative dermatology 2022-05-09 [PMID: 34400195]
• Zastko L, Ra?kov� A, Petrovi?ov� P et al. Evaluation of Calyculin A Effect on ?H2AX/53BP1 Focus Formation and Apoptosis in Human Umbilical Cord Blood Lymphocytes International Journal of Molecular Sciences 2021-05-22 [PMID: 34067339]
• Konkova M, Abramova M, Kalianov A et al. Mesenchymal Stem Cells Early Response to Low-Dose Ionizing Radiation Frontiers in Cell and Developmental Biology 2020-12-14 [PMID: 33381502]
• Jakl L, Markova E et al. Biodosimetry of Low Dose Ionizing Radiation Using DNA Repair Foci in Human Lymphocytes. Genes (Basel) 2020-04-01 [PMID: 31947954]
• Lagund in D, Hu W, Law H et al. Delineating the role of FANCA in glucose-stimulated insulin secretion in beta cells through its protein interactome PLoS ONE 2019-08-28 [PMID: 31461451]
• Parijat Senapati, Hiroyuki Kato, Michael Lee, Amy Leung, Christine Thai, Angelica Sanchez, Emily J. Gallagher, Derek LeRoith, Victoria L. Seewaldt, David K. Ann, Dustin E. Schones Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer Epigenetics & Chromatin 2019-07-17 [PMID: 31315653]
• Tran TQ, Ishak Gabra MB, Lowman XH et al. Glutamine deficiency induces DNA alkylation damage and sensitizes cancer cells to alkylating agents through inhibition of ALKBH enzymes PLoS Biol. 2017-11-01 [PMID: 29107960]
• Sergeeva VA, Ershova ES, Veiko NN et al. Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response Oxid Med Cell Longev 2017-09-14 [PMID: 28904740]
• Giusti L, Gabriele M, Penno G et al. A fermented whole grain prevents lipopolysaccharides-induced dysfunction in Human Endothelial Progenitor Cells. downloads.hindawi.com. 2017-02-20 [PMID: 28386305]
• Singh R, Braga M, Reddy ST et al. Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues. Endocrinology 2017-05-01 [PMID: 28324027]
• Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016-01-14 [PMID: 26735018]
• Somsedikova, A, MarkovA, E Et al. Constitutive 53BP1/gamma H2AX foci are increased in cells of ALL patients dependent on BCR-ABL and TEL-AML1 preleukemic gene fusions. Neoplasma 2014-09-24 [PMID: 25244981]
• Piskounova E, Polytarchou C, Thornton JE et al. Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms. Cell. 2011-11-23 [PMID: 22118463]
• Huang Y, Ratovitski EA. Phosphorylated TP63 Induces Transcription of RPN13, Leading to NOS2 Protein Degradation. J Biol Chem. 2010-10-19 [PMID: 20959455]
• Huang Y, Chuang AY, Romano RA et al. Phospho-DeltaNp63alpha/NF-Y protein complex transcriptionally regulates DDIT3 expression in squamous cell carcinoma cells upon cisplatin exposure. Cell Cycle 2010-01-15 [PMID: 20023394]
• Minoda Y, Saeki K, Aki D et al. A novel Zinc finger protein, ZCCHC11, interacts with TIFA andmodulates TLR signaling. Biochem Biophys Res Commun 2006-06-09 [PMID: 16643855]

Bioinformatics

• Gene Symbol: TUT4
• Entrez:
  • 23318(Human)
• Uniprot:
  • Q5TAX3(Human)