ZBTB4 Antibody - BSA Free

Western Blot: ZBTB4 Antibody [NBP1-76517] - SK-N-SH cell lysate with ZBTB4 antibody at 1 ug/mL.

Immunohistochemistry: ZBTB4 Antibody - BSA Free [NBP1-76517] - Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ZBTB4 Antibody - BSA Free [NBP1-76517] - Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZBTB4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4. The immunogen is located within amino acids 790 - 840 of ZBTB4. Amino Acid Squence: AGTRPGDVKEEAPQEMQV
• Specificity: At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  ZBTB4 Peptide (NBP1-76517PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZBTB4 Antibody - BSA Free

• KAISO-L1
• zinc finger and BTB domain containing 4

Background

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZBTB4 Antibody (NBP1-76517)(1)

Publication using NBP1-76517

• Scott WA, Dhanji EZ, Dyakov BJA et al. ATRX proximal protein associations boast roles beyond histone deposition PLOS Genetics 2021-11-15 [PMID: 34780483]

Bioinformatics

• Gene Symbol: ZBTB4
• Uniprot:
  • EAW90185(Human)