ZAP70 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

ZAP70 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human ZAP70
  Phe10-Cys102
  Accession # P43403
• Specificity: Detects human ZAP70 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZAP70 Antibody [Alexa Fluor® 405]

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

ZAP70 (Zeta-associated protein 70 kDa; also Syk-related tyrosine kinase) is a 68-70 kDa member of the SYK subfamily, tyrosine kinase family of molecules. It is found in immature thymocytes, plus T cells and NK cells, and is essential for TCR-mediated IL-2 production, plus the development of CD8+ T cells. Human ZAP70 is 619 amino acids (aa) in length. It contains two SH2 domains (aa 10-102 and 162-254), a regulatory interdomain region (aa 255-337), and a C-terminal tyrosine kinase domain (aa 338-539). The two SH2 domains bind to phosphorylated tyrosines on the CD3  zeta -chain following TCR activation. This activates the ZAP70 kinase domain with the initiation of downstream signaling via SLP76 and LAT. There are four potential splice variants of ZAP70. One termed TZK is 37 kDa in size, and characterized by an alternative start site at Met308. This is expressed in very early thymocytes. Two others show an alternative start site 88 aa upstream of the standard site, with one of these variants also showing a deletion of aa 495-541. A fourth variant contains an eight aa substitution for aa 1-134. Over aa 10-102, human ZAP70 shares 98% aa identity with mouse ZAP70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.