ZAP70 Antibody (JU08-39)

Western Blot: ZAP70 Antibody (JU08-39) [NBP2-75719] - Analysis of ZAP70 on Jurkat cell lysates with Rabbit anti-ZAP70 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (JU08-39) [NBP2-75719] - Staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ZAP70 Antibody (JU08-39) [NBP2-75719] - Analysis of paraffin-embedded human spleen tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.

Flow Cytometry: ZAP70 Antibody (JU08-39) [NBP2-75719] - Analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Western Blot: ZAP70 Antibody (JU08-39) [NBP2-75719] - Analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (JU08-39) [NBP2-75719] - Staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ZAP70 Antibody (JU08-39) [NBP2-75719] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.

Product Discontinued

ZAP70 Antibody (JU08-39) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ZAP70 aa 570-619 / 619. (SwissProt: P43403 Human)
• Localization: Cytoplasm. Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ZAP70
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ZAP70 Antibody (JU08-39)

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZAP70