ZAP70 Antibody (11H40)

Western Blot: ZAP70 Antibody (11H40) [NBP1-19190] - Analysis of whole cell lysate (20ug (Lane 2 = Jurkat, Lane 3 = Rat Thymus)) and recombinant human Zap-70 (GST-tagged; Lane 4) with anti-Zap-70 mAb used at 1ug/ml.

Immunohistochemistry: ZAP70 Antibody (11H40) [NBP1-19190] - Human tonsil tissue stained with Zap-70, mAb (11H40) at 20ug/ml.

Immunohistochemistry: ZAP70 Antibody (11H40) [NBP1-19190] - Human thymus tissue stained with Zap-70, mAb (11H40) at 20ug/ml.

Product Discontinued

ZAP70 Antibody (11H40) Summary

• Immunogen: Recombinant full length human Zap-70
• Specificity: Zap-70 (11H40)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZAP70
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: In PBS, pH 7.2 and 50% glycerol.
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for ZAP70 Antibody (11H40)

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

Zeta-associated protein 70 (Zap-70) is a cytoplasmic protein tyrosine required for T cell antigen receptor (TCR) signaling and development. ZAP-70 interacts with the TCR by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZAP70 Antibody (NBP1-19190)(1)

Publication using NBP1-19190

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: ZAP70
• Entrez:
  • 7535(Human)
  • 301348(Rat)