Recombinant Human YOD1 His Protein

SDS-Page: Recombinant Human YOD1 His Protein [NBP2-22955] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human YOD1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-348 of Human YOD1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: YOD1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 40.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human YOD1 His Protein

• DKFZp451J1719
• DUBA8
• DUBA-8
• DUBA8hsHIN7
• EC 3.1.2
• EC 3.4.19.12
• HIN7
• HIN-7
• HIV-1-induced protease 7
• HsHIN7
• OTU domain-containing protein 2
• OTUD2
• OTUD2OTU domain containing 2
• PRO0907
• ubiquitin thioesterase OTU1
• YOD1 OTU deubiquinating enzyme 1 homolog ( yeast)
• YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• YOD1

Background

YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: YOD1