YOD1 Antibody

Images

 
Western Blot: YOD1 Antibody [NBP2-29993] - Anti-YOD1 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at ...read more
Immunocytochemistry/ Immunofluorescence: YOD1 Antibody [NBP2-29993] - (C-term)(NBP2-29993) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear ...read more
Immunohistochemistry: YOD1 Antibody [NBP2-29993] - Formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the ...read more
Western Blot: YOD1 Antibody [NBP2-29993] - K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).
Western Blot: YOD1 Antibody [NBP2-29993] - Anti-YOD1 Antibody (C-term) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.92 mg/ml

Order Details

YOD1 Antibody Summary

Immunogen
This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-347 amino acids from the C-terminal region of human YOD1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YOD1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:100
Theoretical MW
38.322 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Chicken and Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.92 mg/ml
Purity
Protein A purified

Alternate Names for YOD1 Antibody

  • DKFZp451J1719
  • DUBA8
  • DUBA-8
  • DUBA8hsHIN7
  • EC 3.1.2
  • EC 3.4.19.12
  • HIN7
  • HIN-7
  • HIV-1-induced protease 7
  • HsHIN7
  • OTU domain-containing protein 2
  • OTUD2
  • OTUD2OTU domain containing 2
  • PRO0907
  • ubiquitin thioesterase OTU1
  • YOD1 OTU deubiquinating enzyme 1 homolog ( yeast)
  • YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
  • YOD1

Background

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YOD1
Entrez
Uniprot