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XPO6 Antibody

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Western Blot: XPO6 Antibody [NBP2-20925] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:3000.
Western Blot: XPO6 Antibody [NBP2-20925] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: XPO6 Antibody [NBP2-20925] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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XPO6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human XPO6. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPO6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for XPO6 Antibody

  • Exp6
  • exportin 6
  • exportin-6
  • FLJ22519
  • KIAA0370EXP6
  • RAN binding protein 20
  • Ran-binding protein 20
  • RANBP20

Background

Exportins, such as XPO6, recruit cargo in the nucleoplasm in the presence of RAN (MIM 601179)-GTP and form ternary export complexes. These complexes are transported through nuclear pore complexes to the cytoplasm, where GTP is hydrolyzed and the export complex is disassembled.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.
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NTF2 Antibody
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Western Blot: NTF2 Antibody [NBP1-82520] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: NTF2 Antibody [NBP1-82520] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
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Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.
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Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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p21/CIP1/CDKN1A
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Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
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Western Blot: EIF3C Antibody [NB100-511] - eIF3C expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.
Western Blot: EIF3C Antibody [NB100-511] - Detection of Human, Mouse and Rat eIF3S8 using NB 100-511. Samples: A. Whole cell lysate (5, 15 or 50 ug for WB) from HEK293 (human), C2C12 (mouse) and L6 (rat) cells. B. Whole cell lysate (12.5 ug for Input and 1 mg for IP) from HEK293 cells. Immunoprecipitation was also performed using NB 100-510 rabbit anti-eIF3S8 antibody at 5 ug/mg lysate. Chemiluminescence with exposure times of 2-4 minutes.
Western Blot: EIF3C Antibody [NB100-511] - Detection of Human eIF3C/eIF3S8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Immunocytochemistry/Immunofluorescence: C9orf5 Antibody [NBP1-90633] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
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Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.
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Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.
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Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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FMN2 Antibody
NBP2-24615
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PTK9 Antibody
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Western Blot: PTK9 Antibody [NBP1-90297] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
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E2F-1 Antibody
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Western Blot: E2F-1 Antibody (8G9) [NBP2-37474] - Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
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Western Blot: Importin-9 Antibody [NB100-56489] - analysis of Importin-9 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 5 ug/ml.
Immunohistochemistry-Paraffin: Importin-9 Antibody [NB100-56489] - analysis of human brain, cortex using this antibody at 10 ug/ml.
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Publications for XPO6 Antibody (NBP2-20925) (0)

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Bioinformatics

Gene Symbol XPO6
Uniprot