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XPO6 Antibody

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Western Blot: XPO6 Antibody [NBP2-20925] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:3000.
Western Blot: XPO6 Antibody [NBP2-20925] - Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: XPO6 Antibody [NBP2-20925] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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XPO6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human XPO6. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPO6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for XPO6 Antibody

  • Exp6
  • exportin 6
  • exportin-6
  • FLJ22519
  • KIAA0370EXP6
  • RAN binding protein 20
  • Ran-binding protein 20
  • RANBP20

Background

Exportins, such as XPO6, recruit cargo in the nucleoplasm in the presence of RAN (MIM 601179)-GTP and form ternary export complexes. These complexes are transported through nuclear pore complexes to the cytoplasm, where GTP is hydrolyzed and the export complex is disassembled.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. Top panel: Nup98-HoxA9 interacts and sequesters CRM1 onto Nup98-HoxA9 dots. HeLa cells were transfected with the EGFP-Nup98-HoxA9 expressing plasmid. After 24 hr, cells were fixed and stained with an anti-CRM1 antibody. Arrows indicate the cells transfected. Bottom panel: Nup98-HoxA9 ES cells were fixed and co-stained with anti-FLAG (M2) and anti-CRM1 antibodies. Merged image of FLAG (green) and CRM1 (red) is shown. Bar, 5um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NTF2 Antibody
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Immunocytochemistry/Immunofluorescence: NTF2 Antibody [NBP1-82520] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Immunohistochemistry-Paraffin: NTF2 Antibody [NBP1-82520] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: NTF2 Antibody [NBP1-82520] - Staining of human testis shows high expression.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
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Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)
Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
(3 Publications)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
p21/CIP1/CDKN1A
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NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(17 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
(6 Publications)
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Western Blot: EIF3C Antibody [NB100-511] - eIF3C expression in naive and activated (overnight) murine B cells. Image submitted by a verified customer review.
Immunoprecipitation: EIF3C Antibody [NB100-511] - Detection of human eIF3C/eIF3S8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for IP at 6 ug per reaction. eIF3C/eIF3S8 was inefficiently immunoprecipitated by rabbit anti-eIF3C/eIF3S8 antibody from Company B. For blotting immunoprecipitated eIF3C/eIF3S8, NB100-511 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: EIF3C Antibody [NB100-511] - Detection of Human eIF3C/eIF3S8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(1 Review)
(3 Publications)
Species: Hu, Mu
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Fibroblast
Activation Protein al ...
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(1 Review)
(17 Publications)
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Applications: IHC, IP, KO, Simple Western, WB
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Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Protein expression analysis of normal and NPC-deficient cells after HIV-1 infection. Cells were uninfected or infected with VSVG-HIV-1 and harvested 96 h post-infection. NPC2, NPC1, and beta-actin protein expression was detected via Western blotting in uninfected and infected cells. Gag expression was also detected in the infected cells. Image collected and cropped by CiteAb from the following publication (http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-31), licensed under a CC-BY licence.
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(47 Publications)
Species: ChHa, Ha, Hu, Mu, Po, Pm, Rt
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Free
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Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.
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Western Blot: FMN2 Antibody [NBP2-24615] - Analysis of FMN2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24615 at 0.25 ug/ml.
Immunohistochemistry-Paraffin: FMN2 Antibody [NBP2-24615] - Tissue section of human brain using 1:300 dilution of FMN2 antibody. The signal was developed using HRP-labeled secondary and DAB reagent which followed counterstaining of nuclei with hematoxylin. This FMN2 antibody generated a nuclear and cytoplasmic staining primarily in the glial cells.
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Applications: IHC, IHC-P, WB
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Applications: IHC, IHC-P
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Elastase/ELA2 Antibod ...
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Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils .
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - THP-1 human acute monocytic leukemia cell line treated with 3 uM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB9167, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
(1 Review)
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Immunocytochemistry/Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
E2F-1 Antibody
(16G7)
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Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
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Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Analysis of paraffin-embedded Mouse prostate, antibody at 1:150 dilution.
(2 Publications)
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Semaphorin 5A was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Sheep Anti-Human/Mouse/Rat Semaphorin 5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5896) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Semaphorin 5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5896) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 5A at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu, Mu, Rt
Applications: ICC, IHC, WB
Importin-9 Antibody
NB100-56489
Knockout Validated: Importin-9 Antibody [NB100-56489] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunohistochemistry-Paraffin: Importin-9 Antibody [NB100-56489] - Analysis of human brain, cortex using this antibody at 10 ug/ml.
Western Blot: Importin-9 Antibody [NB100-56489] - Analysis of Importin-9 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 5 ug/ml.
Species: Hu, Mu, Pm, RM
Applications: IHC, IHC-P, KO, WB
XPO6 Antibody
NBP2-20925
XPO6 Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol XPO6
Uniprot