XPE/DDB2 Overexpression Lysate

Images

 
Western Blot: DDB2 Overexpression Lysate (Adult Normal) [NBL1-09764] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

XPE/DDB2 Overexpression Lysate Summary

Description

XPE/DDB2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200390

Accession#: NM_000107

Protein Tag: C-MYC/DDK
Gene
DDB2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
47.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for XPE/DDB2 Overexpression Lysate

  • damage-specific DNA binding protein 2 (48kD)
  • damage-specific DNA binding protein 2, 48kDa
  • Damage-specific DNA-binding protein 2
  • DDB p48 subunit
  • DDB2
  • DDBB
  • DNA damage-binding protein 2
  • FLJ34321
  • UV-damaged DNA-binding protein 2
  • UV-DDB 2
  • UV-DDB2
  • xeroderma pigmentosum group E protein
  • XP5
  • XPE/DDB2

Background

This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for XPE/DDB2 Lysate (NBL1-09764) (0)

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Bioinformatics

Gene Symbol DDB2