XPE/DDB2 Antibody (JE16-41)

Western Blot: XPE/DDB2 Antibody (JE16-41) [NBP2-75718] - Analysis of DDB2 on Daudi cell using anti-DDB2 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: XPE/DDB2 Antibody (JE16-41) [NBP2-75718] - Analysis of paraffin-embedded human skin tissue using anti-DDB2 antibody. Counter stained with hematoxylin.

Flow Cytometry: XPE/DDB2 Antibody (JE16-41) [NBP2-75718] - Analysis of A549 cells with DDB2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: XPE/DDB2 Antibody (JE16-41) [NBP2-75718] - Analysis of paraffin-embedded human tonsil tissue using anti-DDB2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: XPE/DDB2 Antibody (JE16-41) [NBP2-75718] - Analysis of paraffin-embedded human liver cancer tissue using anti-DDB2 antibody. Counter stained with hematoxylin.

Product Discontinued

XPE/DDB2 Antibody (JE16-41) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human XPE/DDB2 aa 180-427 / 427. (SwissProt: Q92466 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for XPE/DDB2 Antibody (JE16-41)

• damage-specific DNA binding protein 2 (48kD)
• damage-specific DNA binding protein 2, 48kDa
• Damage-specific DNA-binding protein 2
• DDB p48 subunit
• DDB2
• DDBB
• DNA damage-binding protein 2
• FLJ34321
• UV-damaged DNA-binding protein 2
• UV-DDB 2
• UV-DDB2
• xeroderma pigmentosum group E protein
• XP5
• XPE/DDB2

Background

DDB2, also known as DNA damage-binding protein 2, consists of five isoforms of sizes 47.9 kDa, 26.7 kDa, 17.4 kDa, 40.7 kDa, and 26.8 kDa and is involved in repairing DNA damaged by ultraviolet light. Current research is being conducted on disorders and diseases including immunodeficiency, hepatitis, multiple sclerosis, lung cancer, ovarian cancer, skin cancer, and autosomal recessive disease. The protein has been linked to DNA repair and protein stability pathways where it interacts with CUL4A, DDB1, COPS5, ABL1, and E2F1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XPE/DDB2 Antibody (NBP2-75718)(1)

Publication using NBP2-75718

• Hameed J S F, Devarajan A, M S DP et al. PTEN-negative endometrial cancer cells protect their genome through enhanced DDB2 expression associated with augmented nucleotide excision repair BMC cancer 2023-05-04 [PMID: 37142958] (WB, Human)

Bioinformatics

• Gene Symbol: DDB2