XCR1/CCXCR1 Antibody (1097A) [Phycoerythrin]

Human peripheral blood monocytes were stained with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated Monoclonal Antibody (Catalog # FAB4869F), Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog # FAB3947A) and (B) Rabbit Anti-Human XCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB8571P, filled histogram) or isotype control antibody (Catalog # IC105P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1097A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Phycoerythrin

XCR1/CCXCR1 Antibody (1097A) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human XCR1
  Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
  Accession # P46094
• Specificity: Detects human XCR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XCR1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCR1/CCXCR1 Antibody (1097A) [Phycoerythrin]

• CCXCR1
• CCXCR1XC chemokine receptor 1
• chemokine (C motif) receptor 1
• chemokine XC receptor 1
• G protein-coupled receptor 5
• GPR5
• GPR5chemokine (C motif) XC receptor 1
• G-protein coupled receptor 5
• Lymphotactin receptor
• XCR1

Background

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta . In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8⁺ T cells, NK cells, CD4⁺ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XCR1/CCXCR1 Antibody (FAB8571P)(1)

Publication using FAB8571P

• M Canavan, AM Walsh, V Bhargava, SM Wade, T McGarry, V Marzaioli, B Moran, M Biniecka, H Convery, S Wade, C Orr, R Mullan, JM Fletcher, S Nagpal, DJ Veale, U Fearon Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis JCI Insight, 2018-12-06;3(23):. 2018-12-06 [PMID: 30518680] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: XCR1
• Uniprot:
  • P46094()