WSB1 Overexpression Lysate (Native)

Images

 
Western Blot: WSB1 Overexpression Lysate (Adult Normal) [NBL1-17883] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WSB1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WSB1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205839 and based on accession number NM_015626. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens WD repeat and SOCS box-containing 1 (WSB1), transcript variant 1, mRNA.
Gene
WSB1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
47.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WSB1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WSB1 Overexpression Lysate (Native)

  • DKFZp564B0482
  • SOCS box-containing WD protein SWiP-1
  • SWIP1DKFZp564A122
  • WD repeat and SOCS box containing 1
  • WD repeat and SOCS box-containing 1
  • WD repeat and SOCS box-containing protein 1
  • WSB-1

Background

This gene encodes a member of the WD-protein subfamily. This protein shares a high sequence identity to mouse and chick proteins. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for WSB1 Lysate (NBL1-17883) (0)

There are no publications for WSB1 Lysate (NBL1-17883).
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Bioinformatics

Gene Symbol WSB1