Recombinant Human Wnt3 GST (N-Term) Protein

SDS-Page: Recombinant Human Wnt3 Protein [H00007473-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human Wnt3 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-355 of Human WNT3
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MEPHLLGLLLGLLLGGTRVLAGYPIWWSLALGQQYTSLGSQPLLCGSIPGLVPKQLRFCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHGIDGCDLLCCGRGHNTRTEKRKEKCHCIFHWCCYVSCQECIRIYDVHTCK

• Background: Human WNT3 full-length ORF ( NP_110380.1, 1 a.a. - 355 a.a.) recombinant protein with GST-tag at N-terminal.
• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: WNT3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Application Notes: Use in functional reported in scientific literature (PMID 26108805)
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Wnt3 GST (N-Term) Protein

• INT4
• INT4Proto-oncogene Int-4 homolog
• MGC131950
• MGC138321
• MGC138323
• proto-oncogene Wnt-3
• TETAMS
• wingless-type MMTV integration site family, member 3
• WNT-3 proto-oncogene protein
• WNT3
• Wnt-3

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Wnt3 Recombinant Protein (H00007473-P01)(6)

Publications using H00007473-P01

• Y Park, M Lofton, D Li, MR Rasin Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs Cells, 2021-01-28;10(2):. 2021-01-28 [PMID: 33525513]
• Duan RS, Liu PP, Xi F et al. Wnt3 and Gata4 regulate axon regeneration in adult mouse DRG neurons. Biochem Biophys Res Commun 2018-05-05 [PMID: 29567480]
• Wu Y, Tran T, Dwabe S et al. A83-01 inhibits TGF-B-induced upregulation of Wnt3 and epithelial to mesenchymal transition in HER2-overexpressing breast cancer cells. Breast Cancer Res Treat 2017-03-23 [PMID: 28337662]
• Kraushar ML, Viljetic B, Wijeratne HR et al. Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell Subtypes. J Neurosci. 2015-08-05 [PMID: 26245956]
• Krivega M, Essahib W, Van de Velde H. WNT3 and membrane-associated B-catenin regulate trophectoderm lineage differentiation in human blastocysts Mol. Hum. Reprod. 2015-06-24 [PMID: 26108805] (Func, Human)
• Miranda Carlos J, Braun Lyndsey, Jiang Yuying et al. Aging brain microenvironment decreases hippocampal neurogenesis through Wnt-mediated survivin signaling. Aging Cell 2012-01-01 [PMID: 22404871] (AP, Human)

Bioinformatics

• Gene Symbol: WNT3