Wnt3 Antibody

Western Blot: Wnt3 Antibody [NB100-40793] - HEK293 cell lysate (35 ug protein in RIPA buffer). Primary antibody at 1 ug/ml, 1 hr incubation. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Wnt3 Antibody [NB100-40793] - Paraffin embedded human breast tissue. Antibody at 2 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Wnt3 Antibody Summary

• Description: Novus Biologicals Goat Wnt3 Antibody (NB100-40793) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Wnt3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CGRGHNTRTEKRKEK corresponding to internal region according to NP_110380.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:16000
  • Immunohistochemistry 2-4 ug/ul
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Western Blot 1 - 3 ug/ml
• Application Notes: Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1).
  IHC: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells.
• Control:

  Wnt3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Wnt3 Antibody

• INT4
• INT4Proto-oncogene Int-4 homolog
• MGC131950
• MGC138321
• MGC138323
• proto-oncogene Wnt-3
• TETAMS
• wingless-type MMTV integration site family, member 3
• WNT-3 proto-oncogene protein
• WNT3
• Wnt-3

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt3 Antibody (NB100-40793)(2)

Publications using NB100-40793

• Okamoto M, Inoue K, Iwamura H et al. Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis. FASEB J 2011-10-01 [PMID: 21746862]
• Schmitt AM, Shi J, Wolf AM et al. Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping. Nature 2006-01-05 [PMID: 16280981]

Bioinformatics

• Gene Symbol: WNT3
• Entrez:
  • 7473(Human)
  • 22415(Mouse)
  • 24882(Rat)
• Uniprot:
  • NP_110380.1(Human)