Wnt-5b Antibody - BSA Free

Immunohistochemistry-Paraffin: Wnt-5b Antibody - BSA Free [NB100-90598] - Analysis of anti-WNT5B antibody with human prostate at concentration 5 ug/ml.

• Reactivity: Hu, Pm-Or, Pm
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Wnt-5b Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 14 amino acid peptide from internal region of human Wnt-5b.
• Epitope: internal domain of human
• Specificity: Human WNT5B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (93%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
• Application Notes: IHC: NB100-90598 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..
• Control:

  Wnt-5b Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Gorilla (93%), Marmoset (93%), Canine (86%), Equine (86%), Rabbit (86%), Opossum (86%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Wnt-5b Antibody - BSA Free

• MGC2648
• protein Wnt-5b
• wingless-type MMTV integration site family, member 5B
• WNT-5B protein
• Wnt5b
• Wnt-5b

Background

WNT5B is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt proteins constitute a large family of secreted molecules that are involved in intercellular signalling during development and have been implicated in oncogenesis. Very little is known about the structure of Wnts as they are notoriously insoluble; but they share the following features characteristics of secretory proteins: a signal peptide, several potential N-glycosylation sites and 22 conserved cysteines. The Wnt proteins seem to adhere to the plasma membrane of the secreting cells and are therefore likely to signal over only few cell diameters. Fifteen major Wnt gene families have been identified in vertebrates, with multiple subtypes within some classes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT5B
• Entrez:
  • 81029(Human)
• Uniprot:
  • Q9H1J7(Human)