Wnt-5a Antibody (JU30-30)

Western Blot: Wnt-5a Antibody (JU30-30) [NBP2-75714] - Analysis of Wnt5a on Hela cell lysate using anti-Wnt5a antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Wnt-5a Antibody (JU30-30) [NBP2-75714] - Staining Wnt5a in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Wnt-5a Antibody (JU30-30) [NBP2-75714] - Analysis of paraffin-embedded human lung cancer tissue using anti-Wnt5a antibody. Counter stained with hematoxylin.

Flow Cytometry: Wnt-5a Antibody (JU30-30) [NBP2-75714] - Analysis of SH-SY5Y cells with Wnt5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Discontinued

Wnt-5a Antibody (JU30-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Wnt-5a aa 331-380 / 380. (SwissProt: P41221 Human)
• Localization: Secreted, extracellular space, extracellular matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNT5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Wnt-5a Antibody (JU30-30)

• hWNT5A
• protein Wnt-5a
• wingless-type MMTV integration site family, member 5A
• WNT-5A protein
• Wnt5a
• Wnt-5a

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT5A