Wnt-4 Antibody

Western Blot: Wnt-4 Antibody [NB100-1006] - (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Wnt-4 Antibody [NB100-1006] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Wnt-4 Antibody Summary

• Description: Novus Biologicals Goat Wnt-4 Antibody (NB100-1006) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Wnt-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SNWLYLAKLSSVGS corresponding to internal region according to NP_110388.2.
• Localization: Possibly secreted and associates with the extracellular matrix.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 40+35 kDa band observed in Jurkat lysates (calculated MW of 39.1 kDa band according to NP_110388.2 Negative Control: U937 cell lysate.).
  Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  Wnt-4 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Wnt-4 Antibody

• protein Wnt-4
• SERKAL
• wingless-type MMTV integration site family, member 4
• Wnt4
• Wnt-4

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and DAX1, a gene known to antagonize the testis-determining factor, play a concerted role in both the control of female development and the prevention of testes formation. WNT4 and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-4 Antibody (NB100-1006)(1)

Publication using NB100-1006

• Huguet EL, McMahon JA, McMahon AP et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell. Cancer Res 1994-05-15 [PMID: 8168088]

Bioinformatics

• Gene Symbol: WNT4
• Entrez:
  • 54361(Human)
  • 22417(Mouse)
• Uniprot:
  • NP_110388.(Human)