Wnt-4 Antibody (55025) [Unconjugated]

HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of human breast cancer tissue. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of human breast cancer tissue. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Wnt-4 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Wnt-4 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; <a class=

HAF005). A specific band was detected for Wnt‑4 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF005). A specific band was detected for Wnt‑4 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western blot shows lysates of MCF‑7 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Wnt‑4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Wnt‑4 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western blot shows lysates of MCF‑7 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Wnt‑4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Wnt-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Wnt-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Wnt-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Wnt-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." > DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." title="Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." />

Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) coated on a Clear Polystyrene Microplate (<a class=

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, ICC/IF
• Clone: 55025
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-4 Antibody (55025) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Wnt‑4
  Ile37-Glu76
  Accession # NP_033549
• Specificity: This antibody detects human and mouse Wnt-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Wnt-1, 2b, 5a, 5b, 8a, 8b, 9b, 10a, 10b, 11, 16, recombinant human (rh) Wnt-2, 3a, 7a, or 7b is observed. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: WNT4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-4 Antibody (55025) [Unconjugated]

• protein Wnt-4
• SERKAL
• wingless-type MMTV integration site family, member 4
• Wnt4
• Wnt-4

Background

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-4 Antibody (MAB4751)(6)

Publications using MAB4751

• Yang, L;Ruan, Z;Lin, X;Wang, H;Xin, Y;Tang, H;Hu, Z;Zhou, Y;Wu, Y;Wang, J;Qin, D;Lu, G;Loomes, KM;Chan, WY;Liu, X; NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations Nature communications 2024-01-16 [PMID: 38228611] (Western Blot, IHC, Mouse)
• Leah Sommerfeld, Florian Finkernagel, Julia M. Jansen, Uwe Wagner, Andrea Nist, Thorsten Stiewe et al. The multicellular signalling network of ovarian cancer metastases Clinical and Translational Medicine 2021-11-08 [PMID: 34841720] (Western Blot, Human)
• Sadhana A. Samant, Vinodkumar B. Pillai, Mahesh P. Gupta Skeletal muscle‐specific over‐expression of the nuclear sirtuin SIRT6 blocks cancer‐associated cachexia by regulating multiple targets JCSM Rapid Communications 2021-01-01 [PMID: 34212132]
• MT Shacklefor, DM Rao, EK Bordeaux, HM Hicks, CG Towers, JL Sottnik, S Oesterreic, MJ Sikora Estrogen Regulation of mTOR Signaling and Mitochondrial Function in Invasive Lobular Carcinoma Cell Lines Requires WNT4 Cancers (Basel), 2020-10-12;12(10):. 2020-10-12 [PMID: 33053661] (Western Blot, Human)
• LY Hung, JL Johnson, Y Ji, DA Christian, KR Herbine, CF Pastore, DR Herbert Cell-Intrinsic Wnt4 Influences Conventional Dendritic Cell Fate Determination to Suppress Type 2 Immunity J. Immunol., 2019-06-07;0(0):. 2019-06-07 [PMID: 31175162] (IHC, Mouse)
• Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. BMC Cancer, 2013-11-25;13(0):557. 2013-11-25 [PMID: 24274766] (Western Blot, Human)

Bioinformatics

• Gene Symbol: WNT4
• Uniprot:
  • NP_033549(Mouse)