Wnt-2 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Wnt-2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Wnt‑2
  Thr33-Glu75, Leu222-Arg292
  Accession # P09544
• Specificity: Detects human Wnt-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Wnt‑7a and rhWnt‑7b is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-2 Antibody [Alexa Fluor® 350]

• INT1L1
• INT1L1secreted growth factor
• Int-1-like protein 1
• Int-1-related protein
• IRP
• IRPprotein Wnt-2
• wingless-type MMTV integration site family member 2
• Wnt2
• Wnt-2

Background

Human Wnt-2 is a 35 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 1 Wnt based on its strong transforming activity on C57MG cells. Human Wnt-2 is synthesized as a 360 amino acid (aa) precursor that contains a 335 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature human Wnt-2 is 96% aa identical to mature mouse and rat Wnt-2. Wnt-2 is produced by mammary myoepithelial cells, endometrial epithelial cells, and breast fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.