Wnt-10b Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-10b Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Wnt-10b peptide
  Leu42-Glu82, Arg263-Arg332
  Accession # P48614
• Specificity: Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) Wnt-3a, rmWnt-4, rmWnt-5a, and rmWnt-8a is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT10B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-10b Antibody

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists as two isoforms: a 361 amino acid (aa) long form and a 265 aa short form that lacks a 95 aa internal sequence. Wnt-10b is less than 60% aa identical to other Wnts, but mouse to human, Wnt-10b shows almost complete aa sequence identity at > 97%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT10B
• Uniprot:
  • P48614()