Wnt-10b Antibody (793127) [Unconjugated]

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Wnt-10b Monoclonal Antibody (Catalog # MAB7196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Wnt-10b at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysate of SW480 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Wnt‑10b at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 793127
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-10b Antibody (793127) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Wnt-10b
  Asn29-Lys389
  Accession # O00744
• Specificity: Detects human Wnt-10b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Wnt-6 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: WNT10B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-10b Antibody (793127) [Unconjugated]

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8⁺ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
2. Katoh, M. (2008) Curr. Drug Targets 9:565.
3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
4. Swiss Prot Accession # O00744.
5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
8. Wright, W.S. et al. (2007) Diabetes 56:295.
9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-10b Antibody (MAB7196)(1)

Publication using MAB7196

• Berg R, Rahbek S, Kofod-Olsen E, Holm C, Melchjorsen J, Jensen D, Hansen A, Jorgensen L, Ostergaard L, Tolstrup M, Larsen C, Paludan S, Jakobsen M, Mogensen T T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication. PLoS ONE, 2014-01-03;9(1):e84513. 2014-01-03 [PMID: 24404168]

Bioinformatics

• Gene Symbol: WNT10B
• Uniprot:
  • O00744()