Wnt-10b Antibody (254206)

• Reactivity: Mu
• Applications: WB
• Clone: 254206
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-10b Antibody (254206) Summary

• Immunogen: E. coli-derived recombinant mouse Wnt-10b
  Arg263-Arg332
  Accession # P48614
• Specificity: Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, -3a, -4,
  -5a, -5b, -8a, -11, or -16 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: WNT10B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-10b Antibody (254206)

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Wnt-10b, also known as Wnt-12, is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists in two isoforms that are distinguished by the presence or absence of an internal 95 amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-10b Antibody (MAB2110)(3)

Publications using MAB2110

• Ulrike I. Mödder, Merry Jo Oursler, Sundeep Khosla, David G. Monroe Wnt10b activates the wnt, notch, and NF kappa B pathways in u2os osteosarcoma cells Journal of Cellular Biochemistry 5/1/2011 [PMID: 21321991]
• Fox KE, Colton LA, Erickson PF, Friedman JE, Cha HC, Keller P, MacDougald OA, Klemm DJ Regulation of cyclin D1 and Wnt10b gene expression by cAMP-responsive element-binding protein during early adipogenesis involves differential promoter methylation. J. Biol. Chem., 2008-10-27;283(50):35096-105. 2008-10-27 [PMID: 18957421] (Western Blot, Mouse)
• Cheng SL, Shao JS, Cai J, Sierra OL, Towler DA Msx2 exerts bone anabolism via canonical Wnt signaling. J. Biol. Chem., 2008-05-15;283(29):20505-22. 2008-05-15 [PMID: 18487199] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: WNT10B
• Uniprot:
  • P48614()