WIZ Antibody

Western Blot: WIZ Antibody [NBP1-21059] - Human Cerebellum lysate (A) + Blocking peptide (B) (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence

Flow Cytometry: WIZ Antibody [NBP1-21059] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Antibody at 10 ug/mL. Primary incubation 1hr followed by Alexa Fluor 488 secondary antibody at 1 ug/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

WIZ Antibody Summary

• Description: Novus Biologicals Goat WIZ Antibody (NBP1-21059) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-WIZ Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RSYIQGGRPFTKKFR corresponding to internal region according to NP_067064.2.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WIZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx. 75 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 85.4 kDa according to NP_067064.2). An additional band of unknown identity was also consistently observed at 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23213215).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WIZ Antibody

• widely interspaced zinc finger motifs
• widely-interspaced zinc finger motifs
• Widely-interspaced zinc finger-containing protein
• WIZ zinc finger
• WIZ
• Zinc finger protein 803
• ZNF803
• ZNF803protein Wiz

Background

WIZ may link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved inEHMT1-EHMT2 heterodimer formation and stabilization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WIZ Antibody (NBP1-21059)(2)

Publications using NBP1-21059

• Tirard M, Hsiao HH, Nikolov M et al. In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice Proc Natl Acad Sci U S A 2012-12-03 [PMID: 23213215] (WB, Mouse)
• Rathert P, Dhayalan A, Murakami M et al. Protein lysine methyltransferase G9a acts on non-histone targets. Nat Chem Biol 2008-06-01 [PMID: 18438403]

Bioinformatics

• Gene Symbol: WIZ
• Entrez:
  • 58525(Human)
  • 22404(Mouse)
  • 314598(Rat)
• Uniprot:
  • O95785(Mouse)