Western Blot: WHSC1 Antibody [NBP1-82633] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)
Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody [NBP1-82633] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
Western Blot: WHSC1 Antibody [NBP1-82633] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Recommended dilution: 1:250 - 1:500
Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human small intestine shows cytoplasmic and nuclear positivity in glandular cells.
Hu, Mu, RtSpecies Glossary
WB, ICC/IF, IHC, IHC-P
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
This antibody was developed against Recombinant Protein corresponding to amino acids:SANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGD
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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For IHC-Paraffin HIER pH6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.