WDR77 Antibody

Images

 
Western Blot: WDR77 Antibody [NBP2-20901] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP2-20901] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-20901] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

WDR77 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WDR77. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WDR77
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (87%), Rat (87%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WDR77 Antibody

  • Androgen receptor cofactor p44
  • MEP-50
  • MEP50Nbla10071
  • methylosome protein 50
  • MGC2722
  • p44
  • p44/Mep50
  • RP11-552M11.3
  • WD repeat domain 77
  • WD repeat-containing protein 77

Background

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WDR77
Uniprot