WDR51A Antibody - BSA Free

Western Blot: WDR51A Antibody [NBP1-88008] - Analysis in control (vector only transfected HEK293T lysate) and POC1A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: WDR51A Antibody [NBP1-88008] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: WDR51A Antibody [NBP1-88008] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: WDR51A Antibody [NBP1-88008] - Staining of human rectum shows strong granular cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: WDR51A Antibody [NBP1-88008] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WDR51A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RLLYTLHGHQGPATTVAFSRTGEYFASGGSDEQVMVWKSNFDIVDHGEVTKVPRPPATLASSMGNLPEVDFPVPPGRGRSVESVQSQPQEPVSVPQTLTSTLEH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  WDR51A Protein (NBP1-88008PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for WDR51A Antibody - BSA Free

• DKFZp434C245
• MGC131902
• PIX2
• POC1 centriolar protein homolog A (Chlamydomonas)
• POC1 centriolar protein homolog A
• WD repeat domain 51A
• WD repeat-containing protein 51A
• WDR51A

Background

POC1 centriolar protein homolog A (POC1A), also known as Pix2 and WD repeat-containing protein 51A (WDR51A), is a member of the WD repeat POC1 family. WDR51A functions to control centriole length and duplication. Additionally, WDR51A is required for ciliogenesis and basal body formation. Mutations to the WDR51A gene result in short stature, onychodysplasia, facial dysmorphism, hypotrichosis (SOFT) syndrome, abnormal cellular mitotic mechanisms and impaired ciliogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POC1A