WDFY3 Antibody (2F7C2) - BSA Free

Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against HEK293 (1) and WDFY3 (AA: 3277-3526)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of Hela cells using WDFY3 mouse mAb (green) and negative control (red).

Immunohistochemistry: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis of paraffin-embedded bladder cancer tissues using WDFY3 mouse mAb with DAB staining.

ELISA: WDFY3 Antibody (2F7C2) [NBP3-27152] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: WDFY3 Antibody (2F7C2) [NBP3-27152] - Analysis using WDFY3 mAb against human WDFY3 (AA: 3277-3526) recombinant protein. (Expected MW is 54.2 kDa)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 2F7C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

WDFY3 Antibody (2F7C2) - BSA Free Summary

• Description: Novus Biologicals Mouse WDFY3 Antibody (2F7C2) - BSA Free (NBP3-27152) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human WDFY3 (AA: 3277-3526) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WDFY3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 395 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for WDFY3 Antibody (2F7C2) - BSA Free

• ALFY
• Autophagy-linked FYVE protein
• KIAA0993MGC16461
• WD repeat and FYVE domain containing 3
• WD repeat and FYVE domain-containing protein 3
• ZFYVE25

Background

The WDFY3 gene encodes a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDFY3