WASF3/WAVE3 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

WASF3/WAVE3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human WASF3/WAVE3
  Met1-Lys180
  Accession # Q9UPY6
• Specificity: Detects endogenous human/mouse WASF3/WAVE3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WASF3/WAVE3 Antibody [Alexa Fluor® 488]

• Brush-1
• KIAA0900WASP family Verprolin-homologous protein 3
• Protein WAVE-3
• SCAR3
• SCAR3WASP family protein member 3
• Verprolin homology domain-containing protein 3
• WAS protein family, member 3
• WASF3
• WAVE3
• WAVE3Brush-1
• wisk

Background

WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.