WASF2 [p Tyr150] Antibody

Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] - Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WASF2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

WASF2 [p Tyr150] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-WASF2 (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WASF2. This sequence has high homology with similar regions in rat and mouse WASF2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151). [UniProt# Q9Y6W5]
• Modification: p Tyr150
• Specificity: This antibody was cross-adsorbed to phospho-WASF2 (Tyr-125) and unphosphorylated WASF2 (Tyr-150) peptides before affinity purification using phospho-WASF2 (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa corresponding to phosphorylated WASF2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WASF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for WASF2 [p Tyr150] Antibody

• IMD2
• Protein WAVE-2
• SCAR2suppressor of cyclic-AMP receptor (WASP-family)
• Verprolin homology domain-containing protein 2
• WAS protein family, member 2
• WASP family Verprolin-homologous protein 2
• WAVE2dJ393P12.2
• wiskWASP family protein member 2

Background

WASF2 encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WASF2