VSIG3/IGSF11 Antibody (2637B) [Unconjugated]

Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for VSIG3 at approximately 57 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human and mouse MB tumor cells exhibit in vitro expression of VISTA binding partners and are translationally relevant. (a) Cells gated on live singlets, then further gated based on GFP expression. Histograms (blue) representing in vitro expression of VSIG8 and VSIG3 in human MB cell lines (D283-MED and D425-MED) and our GFP+ mouse medulloblastoma cell line (mCB DNp53 MYC). Fluorescence minus one control represented in gray, and isotype control represented in green. (b) Western blot analysis confirming expression of VSIG3 and VSIG8 and relative absence of VISTA from human and mouse medulloblastoma tumor cell lines (n = 3). Uncropped blots are shown in File S2. (c) Transcriptome analysis of 52 human MB patient samples. X-axis represents quartile distribution of VISTA expression levels: Q1 (lowest 25%), Q2 (next 25%), Q3 (third 25%), and Q4 (highest 25%). Y-axis represents log2 [TPM+1]. Bar graphs representing VISTA transcript (C10orf54) expression correlation with VSIG3, VSIG8, and PSGL1 and LRIG1. Error bars represent standard error of the mean (SEM), and p-values were calculated using a Mann–Whitney test and corrected for multiple comparisons. The asterisks indicate adjusted p-values as follows: * padj HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human and mouse MB tumor cells exhibit in vitro expression of VISTA binding partners and are translationally relevant. (a) Cells gated on live singlets, then further gated based on GFP expression. Histograms (blue) representing in vitro expression of VSIG8 and VSIG3 in human MB cell lines (D283-MED and D425-MED) and our GFP+ mouse medulloblastoma cell line (mCB DNp53 MYC). Fluorescence minus one control represented in gray, and isotype control represented in green. (b) Western blot analysis confirming expression of VSIG3 and VSIG8 and relative absence of VISTA from human and mouse medulloblastoma tumor cell lines (n = 3). Uncropped blots are shown in File S2. (c) Transcriptome analysis of 52 human MB patient samples. X-axis represents quartile distribution of VISTA expression levels: Q1 (lowest 25%), Q2 (next 25%), Q3 (third 25%), and Q4 (highest 25%). Y-axis represents log2 [TPM+1]. Bar graphs representing VISTA transcript (C10orf54) expression correlation with VSIG3, VSIG8, and PSGL1 and LRIG1. Error bars represent standard error of the mean (SEM), and p-values were calculated using a Mann–Whitney test and corrected for multiple comparisons. The asterisks indicate adjusted p-values as follows: * padj < 0.05, ** padj < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39123357), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 2637B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

VSIG3/IGSF11 Antibody (2637B) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VSIG3
  Gly129-Cys138
  Accession # Q5DX21
• Specificity: Detects human VSIG3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 0.2 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSIG3/IGSF11 Antibody (2637B) [Unconjugated]

• Brain and testis-specific immunoglobulin superfamily protein
• BTIGSF
• BT-IgSF
• cancer/testis antigen 119
• CT119
• CXADR like 1
• CXADRL1
• IGSF11
• Igsf13
• immunoglobulin superfamily member 11
• immunoglobulin superfamily, member 11
• MGC35227
• V-set and immunoglobulin domain containing 3
• V-set and immunoglobulin domain-containing protein 3
• VSIG3
• VSIG3brain and testis-specific immunoglobin superfamily protein

Background

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23-136) and one C2-type Ig-like domain (aa 144-234). Over aa 23-245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211-235.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q5DX21(Human)