VRK1 Overexpression Lysate (Native)

Images

 
Western Blot: VRK1 Overexpression Lysate (Adult Normal) [NBL1-17759] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VRK1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

VRK1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210764 and based on accession number NM_003384. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens vaccinia related kinase 1 (VRK1), mRNA.
Gene
VRK1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for VRK1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VRK1 Overexpression Lysate (Native)

  • EC 2.7.11.1
  • MGC117401
  • MGC138280
  • MGC142070
  • PCH1
  • serine/threonine-protein kinase VRK1
  • vaccinia related kinase 1
  • vaccinia virus B1R-related kinase 1
  • Vaccinia-related kinase 1
  • VRK1

Background

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for VRK1 Lysate (NBL1-17759) (0)

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Bioinformatics

Gene Symbol VRK1
Entrez