VRK1 Antibody (1F6) [Alexa Fluor® 405]

• Applications: WB, IHC, ICC/IF
• Clone: 1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

VRK1 Antibody (1F6) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human VRK1
  Met1-Lys396
  Accession # Q99986
• Specificity: Detects human VRK1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Vaccinia-related kinase 1 (VRK1; also serine/threonine-protein kinase VRK1) is a 45 kDa member of the protein kinase superfamily, the CK1 Serine/Threonine protein kinase family, and the VRK subfamily. Human VRK1 is 396 amino acids (aa) in length, and contains one protein kinase domain (aa 37‑317). Human VRK1 shares 91% and 78% aa sequence identity with bovine and mouse VRK1, respectively. VRK1 is widely expressed with high expression in fetal liver, testis and thymus. VRK1 functions as a serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.