VPS39 Antibody - BSA Free

Western Blot: VPS39 Antibody [NBP1-76535] - Rat liver tissue lysate with VPS39 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: VPS39 Antibody [NBP1-76535] - Saos2 Cells, anti-VPS39 primary, Hoescht33342, anti-Rabbit alexa594 secondary. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] - Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 ug/ml. Green: VPS39 antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: VPS39 Antibody - BSA Free [NBP1-76535] - Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] - Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VPS39 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39. Amino Acid Squence: HKHYDRNKDGNKDVYLS
• Specificity: At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS39
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
• Control Peptide:

  VPS39 Peptide (NBP1-76535PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for VPS39 Antibody - BSA Free

• hVam6p
• KIAA0770FLJ21681
• TLP
• TRAP1-like protein
• vacuolar protein sorting 39 (yeast)
• vacuolar protein sorting 39 homolog (S. cerevisiae)
• vam6/Vps39-like protein
• VAM6FLJ46546

Background

VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS39 Antibody (NBP1-76535)(2)

Publications using NBP1-76535

• Shelke GV, Williamson CD, Jarnik M, Bonifacino JS Inhibition of endolysosome fusion increases exosome secretion The Journal of cell biology 2023-06-05 [PMID: 37213076]
• Jackson J, Wischhof L, Scifo E et al. SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells Molecular metabolism 2022-04-19 [PMID: 35452878]

Review for VPS39 Antibody (NBP1-76535) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 03/28/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Saos2 Cells
• Species: Human
• Lot: ab107570

Bioinformatics

• Gene Symbol: VPS39
• Uniprot:
  • AAQ05978(Human)