VPS39 Antibody

Images

 
Western Blot: VPS39 Antibody [NBP1-76535] - Rat liver tissue lysate with VPS39 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: VPS39 Antibody [NBP1-76535] - Mouse liver tissue.
Immunohistochemistry: VPS39 Antibody [NBP1-76535] - Staining of VPS39 in human liver tissue with VPS39 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: VPS39 Antibody [NBP1-76535] - Saos2 Cells, anti-VPS39 primary, Hoescht33342, anti-Rabbit alexa594 secondary. Image from verified customer review.
Immunohistochemistry-Paraffin: VPS39 Antibody [NBP1-76535] - Mouse liver tissue with VPS39 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

VPS39 Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39. Amino Acid Squence: HKHYDRNKDGNKDVYLS
Specificity
At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS39
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5 ug/ml
Control Peptide
VPS39 Peptide (NBP1-76535PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76535 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for VPS39 Antibody

  • hVam6p
  • KIAA0770FLJ21681
  • TLP
  • TRAP1-like protein
  • vacuolar protein sorting 39 (yeast)
  • vacuolar protein sorting 39 homolog (S. cerevisiae)
  • vam6/Vps39-like protein
  • VAM6FLJ46546

Background

VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS39 Antibody (NBP1-76535) (0)

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Review for VPS39 Antibody (NBP1-76535) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76535:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence VPS39 NBP1-76535
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4
reviewed by:
Verified Customer
IF Human 03/28/2014
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Summary

ApplicationImmunofluorescence
Sample TestedSaos2 Cells
SpeciesHuman
Lotab107570

Blocking

Blocking DetailsBlocked in TBST at room temperature for 1 hour

Primary Anitbody

Dilution Ratio1:5000 in TBST, 1 hour at room temperature

Secondary Antibody

Secondary Descriptionanti-rabbit Alexa594
Secondary Manufacturer Cat#ab150092
Secondary Concentration1:1000 in TBST

Details

Detection NotesOpera high-throughput confocal microscope, 300ms exposure
Fixation DetailsFixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, permeabilized with TBST at room temperature for 1h
Wash DescriptionTBST, 3 washes at 5 minutes each

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS39 Antibody (NBP1-76535) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for VPS39 Antibody (NBP1-76535)

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Verified Customer
03/28/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol VPS39
Uniprot