VPS33B Antibody

Images

 
Western Blot: VPS33B Antibody [NBP2-20874] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: VPS33B Antibody [NBP2-20874] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.97 mg/ml

Order Details

VPS33B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS33B. The exact sequence is proprietary.
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Peripheral membrane protein; Cytoplasmic side
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS33B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus tropicalis (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.97 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS33B Antibody

  • FLJ14848
  • hVPS33B
  • vacuolar protein sorting 33 homolog B (yeast)
  • vacuolar protein sorting 33B (yeast homolog)
  • vacuolar protein sorting 33B
  • vacuolar protein sorting-associated protein 33B

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VPS33B
Uniprot