Recombinant Human VPS24 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human VPS24 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-222 of Human VPS24

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CHMP3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human VPS24 Protein

  • CGI-149
  • CHMP325.1 protein
  • Chromatin-modifying protein 3
  • hVps24
  • member 3,25.1
  • NEDFFLJ38114
  • vacuolar protein sorting 24 (yeast)
  • vacuolar protein sorting 24 homolog (S. cerevisiae)

Background

VPS24, also known as Charged multivesicular body protein 3(CHMP3), belongs to the vacuolar sorting protein family and function as chromatin modifying proteins. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Recombinant human VPS24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CHMP3