Recombinant Human Vitronectin His Protein

SDS-Page: Recombinant Human Vitronectin Protein [NBP2-77511] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Discontinued

Recombinant Human Vitronectin His Protein Summary

• Description: A bioactive recombinant protein with a C terminal His-tag and corresponding to the amino acids 20-478 of Human Vitronectin.
  
  

  Source: Baculovirus

  Amino Acid Sequence: ADPDQESCKG RCTEGFNVDK KCQCDELCSY YQSCCTDYTA ECKPQVTRGD VFTMPEDEYT VYDDGEEKNN ATVHEQVGGP SLTSDLQAQS KGNPEQTPVL KPEEEAPAPE VGASKPEGID SRPETLHPGR PQPPAEEELC SGKPFDAFTD LKNGSLFAFR GQYCYELDEK AVRPGYPKLI RDVWGIEGPI DAAFTRINCQ GKTYLFKGSQ YWRFEDGVLD PDYPRNISDG FDGIPDNVDA ALALPAHSYS GRERVYFFKG KQYWEYQFQH QPSQEECEGS SLSAVFEHFA MMQRDSWEDI FELLFWGRTS AGTRQPQFIS RDWHGVPGQV DAAMAGRIYI SGMAPRPSLA KKQRFRHRNR KGYRSQRGHS RGRNQNSRRP SRATWLSLFS SEESNLGANN YDDYRMDWLV PATCEPIQSV FFFSGDKYYR VNLRTRRVDT VDPPYPRSIA QYWLGCPAPG HLHHHHHH

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of B16-F10 mouse melanoma cells. When cells are added to VTN coated plates 5ug/ml. This effect is more to 50%.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: VTN
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 0.1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 53.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol.
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Vitronectin His Protein

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VTN