Human Vitronectin ELISA Kit (Chemiluminescence)

ELISA: Human Vitronectin ELISA Kit (Chemiluminescence) [NBP3-07112] - Samples were spiked with high concentrations of Human Vitronectin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Vitronectin ELISA Kit (Chemiluminescence) [NBP3-07112] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.94 - 60 ng/mL
• Sensitivity: 0.56 ng/mL

Human Vitronectin ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human Vitronectin and analogues was observed.
• Standard Curve Range: 0.94 - 60 ng/mL
• Sensitivity: 0.56 ng/mL
• Assay Type: Sandwich-CLIA
• Spike Recovery: 92 - 116%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: VTN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Vitronectin ELISA Kit (Chemiluminescence)

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Monoclonal antibody BV1 recognizes human vitronectin. Vitronectin is an abundant glycoprotein (~75 kDa), consisting of 459 amino acids. About one third of the protein molecular mass is composed of carbohydrates. Vitronectin is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, it inhibits cytolysis by the complement C5b-9 complex, and it modulates antithrombin III-thrombin action in blood coagulation. The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain (347-459) also with hemopexin homology. The Somatomedin B domain binds to Plasminogen Activator Inhibitor-1 (PAI-1) and is responsible for PAI-1 stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Amino acid 45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix. Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Vitronectin ELISA Kit (NBP3-07112). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VTN