VHR/DUSP3 Antibody (237020) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Clone: 237020
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

VHR/DUSP3 Antibody (237020) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human, mouse, and rat VHR in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VHR/DUSP3 Antibody (237020) [Alexa Fluor™ Plus 647]

• dual specificity phosphatase 3
• dual specificity protein phosphatase 3
• Dual specificity protein phosphatase VHR
• DUSP3
• EC 3.1.3.16
• EC 3.1.3.48
• Vaccinia H1-related phosphatase
• vaccinia virus phosphatase VH1-related
• VHR
• VHRserine/threonine specific protein phosphatase

Background

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

1. Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
2. Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
3. Alonso, A. et al. (2003) Nat. Immunol. 4:44.
4. Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
5. Yuvaniyama, J. et al. (1996) Science 272:1328.
6. Ueda, K. et al. (2002) FEBS Lett. 525:48.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.