VGLUT2 Antibody (N29/29) [FL550] - Chimeric

• Reactivity: Hu, Mu, Rt, Ch
• Applications: ICC/IF, IHC
• Clone: N29/29
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: FL550
• Concentration: 0.5 mg/ml

VGLUT2 Antibody (N29/29) [FL550] - Chimeric Summary

• Description: FL550 Excitation: 550 nm, Emission: 575nm.
  Each order is a unique lot. For specialized requirements, please inquire for available options.
  
  This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGLUT2 (Uniprot#: Q9JI12) produced recombinantly in E. Coli
• Specificity: Does not cross-react with VGlut1 or VGlut3
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: SLC17A6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Electric Ray

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for VGLUT2 Antibody (N29/29) [FL550] - Chimeric

• differentiation-associated BNPI
• differentiation-associated Na(+)-dependent inorganic phosphate cotransporter
• differentiation-associated Na-dependent inorganic phosphate cotransporter
• DNPI
• solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• solute carrier family 17 member 6
• vesicular glutamate transporter 2
• VGLUT2

Background

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC17A6