VGLL4 Antibody - BSA Free

Western Blot: VGLL4 Antibody [NBP2-93539] - Analysis of extracts of OVCAR3 cells, using VGLL4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Immunohistochemistry-Paraffin: VGLL4 Antibody [NBP2-93539] - Immunohistochemistry of paraffin-embedded Rat testis using VGLL4 Rabbit pAb (NBP2-93539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: VGLL4 Antibody [NBP2-93539] - Immunohistochemistry of paraffin-embedded Mouse brain using VGLL4 Rabbit pAb (NBP2-93539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: VGLL4 Antibody [NBP2-93539] -Rat testis using Vgll4 Rabbit pAb at dilution of 1:100 (20x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VGLL4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VGLL4 Antibody - BSA Free (NBP2-93539) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of mouse Vgll4 (NP_808351.1). MLFMKMDLLNYQYLDKMNNNIGVLCYEGEASLRGEPRMQTLPVASALSSHRTGPPPISPSKRKFSMEPGDKDLDCENDHVSKMSRIFSPHLNKTVNGDCRRDPRERSRSPIERAAAPAVSLHGGHLYASLPSLMEQPLALTKNSSDTGRSAVERQQNRPSVITCASAGARNCNLSHCPIAHSGCSAPGSASYRRPPSATATCDPVVEEHFRRSLGKNYKEPEPAPNSVSITGSVDDHFAKALGDTWLQIKAAKDSASSSPESASRRGQPASPTAHMVSHSHSPSVVS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VGLL4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for VGLL4 Antibody - BSA Free

• KIAA0121transcription cofactor vestigial-like protein 4
• vestigial like 4 (Drosophila)
• Vestigial-like 4
• VGL-4

Background

VGLL4 may act as a specific coactivator for the mammalian TEFs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: VGLL4