VGF Antibody (13#) - BSA Free

Western Blot: VGF Antibody (13#) [NBP3-41613] - Sample: Lane1: Rat Placenta lysate; Lane2: U87MG cell lysate Primary Ab: 3ug/ml NBP3-41613; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Immunohistochemistry-Paraffin: VGF Antibody (13#) [NBP3-41613] - DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 30ug/ml NBP3-41613; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Western Blot: VGF Antibody (13#) [NBP3-41613] - Sample: Lane1: Rat Placenta lysate; Lane2: U87MG cell lysate Primary Ab: 3ug/ml NBP3-41613; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: 13#
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VGF Antibody (13#) - BSA Free Summary

• Description: Novus Biologicals Mouse VGF Antibody (13#) - BSA Free (NBP3-41613) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunized with recombinant Human VGF (Uniprot#: O15240; Asp330-Pro449 (Uniprot#: O15240)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VGF
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-30ug/mL
  • Immunohistochemistry-Paraffin 5-30ug/mL
  • Western Blot 0.01-3ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS, pH7.4, 50% glycerol
• Preservative: 0.05% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for VGF Antibody (13#) - BSA Free

• neuro-endocrine specific protein VGF
• neurosecretory protein VGF
• VGF nerve growth factor inducible
• VGF

Background

Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VGF