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Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

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ELISA: VEGF Receptor 3 ELISA Kit 96Tests [NBP1-84822]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (citrate), buffered solution
Standard Curve Range
0.6 - 40.0 ng/mL
Sensitivity
0.03 ng/mL

Order Details

Human VEGFR3/Flt-4 ELISA Kit (Colorimetric) Summary

Description
The human VEGF-R3/FLT-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R3/FLT-4
Specificity
The assay detects both natural and recombinant human VEGF-R3/ FLT-4. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-R3/FLT-4 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 and VEGF-R2/KDR. Interference was detected for VEGF-C at concentrations > 7 ng/ml, and not for VEGF-D.
Standard Curve Range
0.6 - 40.0 ng/mL
Sensitivity
0.03 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.0%
Intra-Assay
CV% < 5.7%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FLT4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R3/FLT-4
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human VEGF-R3/FLT-4 monoclonal antibody
  5. Human VEGF-R3/FLT-4 Standard lyophilized, 80 ng/ml upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

FLT4, a VEGF Receptor type protein kinase, is an endothelial cell-specific receptor. Binding of the extracellular domain of FLT4 to the vascular endothelial growth factor-related protein VEGF-C stimulates tyrosine phosphorylation and mitogenesis of endothelial cells. FLT4 (-/-) mice have been shown to have defective blood vessel development in early embryos and to experience cardiovascular failure at embryonic day 9.5. A mutation in the FLT4 gene has been implicated in human hereditary lymphedema type I. Similarly, a mutation in the conserved catalytic domain of FLT4, in close proximity to the FLT4 mutations in human primary lymphedema, has been linked to the Chy mouse mutant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FLT4