Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

ELISA: VEGF Receptor 3 ELISA Kit 96Tests [NBP1-84822]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate), buffered solution
• Standard Curve Range: 0.6 - 40.0 ng/mL
• Sensitivity: 0.03 ng/mL

Human VEGFR3/Flt-4 ELISA Kit (Colorimetric) Summary

• Description: The human VEGF-R3/FLT-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R3/FLT-4
• Specificity: The assay detects both natural and recombinant human VEGF-R3/ FLT-4. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-R3/FLT-4 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 and VEGF-R2/KDR. Interference was detected for VEGF-C at concentrations > 7 ng/ml, and not for VEGF-D.
• Standard Curve Range: 0.6 - 40.0 ng/mL
• Sensitivity: 0.03 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.0%
• Intra-Assay: CV% < 5.7%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FLT4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

• EC 2.7.10
• EC 2.7.10.1
• FLT4
• Flt-4
• fms-related tyrosine kinase 4
• LMPH1A
• PCLFLT41
• soluble VEGFR3 variant 1
• soluble VEGFR3 variant 2
• soluble VEGFR3 variant 3
• Tyrosine-protein kinase receptor FLT4
• vascular endothelial growth factor receptor 3
• VEGF R3
• VEGFR3
• VEGFR-3
• VEGFR3Fms-like tyrosine kinase 4

Background

FLT4, a VEGF Receptor type protein kinase, is an endothelial cell-specific receptor. Binding of the extracellular domain of FLT4 to the vascular endothelial growth factor-related protein VEGF-C stimulates tyrosine phosphorylation and mitogenesis of endothelial cells. FLT4 (-/-) mice have been shown to have defective blood vessel development in early embryos and to experience cardiovascular failure at embryonic day 9.5. A mutation in the FLT4 gene has been implicated in human hereditary lymphedema type I. Similarly, a mutation in the conserved catalytic domain of FLT4, in close proximity to the FLT4 mutations in human primary lymphedema, has been linked to the Chy mouse mutant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VEGFR3/Flt-4 ELISA Kit (NBP1-84822). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FLT4